English translation for "dna extraction"

dna提取

Related Translations:

process of mercury extraction:  提汞法

线粒体dna:  mitochondrial dna

proviral dna:  前簿dna,原簿dna

genomic dna:  基因组dna,即染色体dna即染色体dna

载体dna:  vector dna

nonrepetitive dna:  非重复dna

dna电泳:  dna electrophoresis

dna heterozygous:  dna杂合子的

dna profiling:  dna纹印测试

oc dna:  开环dna,开环脱氧核糖核酸

Example Sentences:

	1.	Comparison research of methods of dna extraction from grape 提取方法的比较研究
	2.	A simple and rapid method for genome dna extraction from arabidopsis 以气相色谱法
	3.	Comparison of different dna extraction methods for activated sludge 不同提取方法的比较
	4.	After plasmid and cherosome dna extraction and purification , the gyra of 16 isolates of salmonella gene was amplified by polymerase chain reaction ( pcr ) 测定了16株致病性沙门氏菌对12种抗菌药物的最低抑菌浓度( mic ) ，依照美国临床实验室标准委员会( nccls )标准( 1990 )判定菌株耐药性。
	5.	Dna extraction from different specimens is the foundation of conservation genetics . it is difficult to extract total dna from tannage of chinese alligator with usual method 利用不同的实验材料提取适合研究的dna是保护遗传学研究的基础，而对于鞣制皮革，用常规的dna提取方法很难提到适合于研究的dna样品。
	6.	The shotgun method was only used with b . subtilis hn503 . the optimun conditions for total dna extraction , enzyme digest , ligation and transformation were studied the results showed that the most important factor was transformation efficiency . since the concentration of ligation products was low in common 在已知基因序列的条件下用pcr和rt一cr克隆基因时，对pcr和rt一pcr的反应条件、产物的回收、回收产物的酶切、酶连和转化等条件进行了一系列的比较研究。
	7.	Among the three methods used in the experiment of dna extraction , only ctab , adding pvp in the dna isolation step , had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process . pcr amplifications were performed in a final volume of 25 mm3 containing 0 . 5 units taq polymerase , 2 通过在抽提缓冲液加入2的pvp 、并用异丙醇和乙醇沉淀基因组dna等改良措施， ctab法能避开大量纤维、多糖的影响，有效地从不同属种的棕榈科植物的幼嫩叶片中提取并纯化了适合rapd的基因组dna 。
	8.	After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。
	9.	One small captive population of amur tigers ( panthera tigris altica ) , which had 14 - year captive history since its foundation in 1986 , was selected for such population genetic researches in this paper , which contained three experimental parts as : ( 1 ) dna extraction from shed hairs . three methods , which were chelex , ionic detergent and non - ionic detergent , were respectively used to digest the shed hairs of one amur tiger 本文利用chelex 、离子型和无离子型分解液三种方法对在东北虎( pantheratigrtsaltica )换毛期采集的自然脱落毛发进行了dna提取，并对部分毛发分解原液进行蛋白质沉淀，对于毛发分解残留物也进行了核细胞分解与蛋白质沉淀再处理，对上述提取产物进行rapd扩增。
	10.	Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment , three methods were used to extract genomic dna of bacillus subtilis , i . e . low melting - point agarose embedding method , sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method . the genomic dna of bacillus subtilis were gained by these methods , and the operated programs of the methods were improved . the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods 大片段基因组dna的提取为了获得用于pcr扩增(长距离pcr扩增和分段pcr扩增)的高纯度、大片段(至少为pcr产物长度的4倍)的dna模板，应用三种方法：低熔点琼脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和细菌基因组dna提取试剂盒法，分别提取获得了枯草杆菌基因组dna ，并对3种方法的操作程序进行了不同程度的改进，结果表明：低熔点琼脂糖包埋法提取的基因组dna片段明显大于后两种方法，采用0 . 5琼脂糖凝胶电泳3h ，仍然跑不出加样孔。

Similar Words：

"dna encapsulation" English translation, "dna evolution" English translation, "dna excision repair" English translation, "dna exogenous pigment" English translation, "dna extrachromosomal inheritance" English translation, "dna extractor" English translation, "dna extranuclear gene" English translation, "dna feeding" English translation, "dna fiber autoradiography" English translation, "dna fiber fish" English translation